O-039 Novel sperm centrosome function, structure, and composition during fertilization

Abstract Sperm centrioles play a critical role in sperm differentiation and embryo development, they are implicated infertility miscarriages. However, the precise way function inherited during fertilization mammals remains unresolved. A key reason for this lack of understanding is that many proteins only transiently present centriole because process known as Centrosome Reduction exhibit dramatic species-specific adaptions. It was long widely accepted structural degeneration functional inactivation occur at one or two mammals, resulting vestigial remnant. This led to belief human spermatozoa have (the Proximal Centriole, PC). While happens mice, our studies suggest present, both essential non-murine mammals. Distal Centriole (DC) has highly atypical structure composition, while PC modestly modified. These findings us new model: Remodeling, which states preexisting spermatid centrioles, along with their pericentriolar material (PCM), go through compositional transformation remodeled PCM functions dynamic basal complex (DBC) spermatozoon precursor embryonic centrioles. crucial mystery remaining is: what centrioles' functions? To answer question, we study remodeling infertile men rabbit mutants partially disabled Trial registration number -